Escola Superior de Conservació i Restauració de Bés Culturals de Catalunya
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Efficiency of antibiotics and gamma irradation in eliminating Streptomyces strains isolated from paintings of ancient Egyptian tombs.

Contributor(s): ABDEL-HALIEM, Mahmoud E.F | ALI, Mona F | GHALY, Mohamed F | SAKR, Akmal AMaterial type: ArticleArticleDescription: 6 pISBN: 1296-2074Subject(s): 16S rRNA gene | Atzurita | Azurita | Azurite | Estreptomicetos | Estreptomicets | Gamma irradation | Gen 16S rRNA | Hematita | Hematite | Irradació gamma | Irradiación gamma | Lamp black | Limonita | Limonite | Malachite | Malaquita | Negre fum | Negro humo | Oserkon II | Streptomyces | Tanis | Tell Basta In: Journal of Cultural Heritage 3 14 1, 45-50Abstract: Forty-six Streptomyces strains were isolated from paintings and stone surfaces from Tell Basta and Tanis tombs (80km south-east Cairo, Egypt). Eight of these strains were selectec to determine their sensitivity against 13 antibiotics. In general, high levels of resistance could be observed. Gentamycin, spiramycin and doxycycline wer the most effective antibiotics against the majority of strains under study. Due to the observed antibiotic resistances, gamma irradation was studied as a possible alternative to inhibit microbial growth. Isolated bacteria were exposed to different doses of gamma irradation (5, 10, 15, 20 and 25 kGy). The growth of all Streptomyces isolates except S. canarius was completely inhibed at 25 kGy. The applied doses of gamma irradation did not cause any observable alterations or colour changes to pigments and binding media (arabic gum, animal glue and egg-yolk) used in the paintings.
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Article de revista Article de revista Biblioteca de l' Escola Superior Conservació i Restauració de Bens Culturals de Catalunya
Journal of Cultural Heritage 3 (Browse shelf(Opens below)) Available R: 2986 Art-106

Forty-six Streptomyces strains were isolated from paintings and stone surfaces from Tell Basta and Tanis tombs (80km south-east Cairo, Egypt). Eight of these strains were selectec to determine their sensitivity against 13 antibiotics. In general, high levels of resistance could be observed. Gentamycin, spiramycin and doxycycline wer the most effective antibiotics against the majority of strains under study. Due to the observed antibiotic resistances, gamma irradation was studied as a possible alternative to inhibit microbial growth. Isolated bacteria were exposed to different doses of gamma irradation (5, 10, 15, 20 and 25 kGy). The growth of all Streptomyces isolates except S. canarius was completely inhibed at 25 kGy. The applied doses of gamma irradation did not cause any observable alterations or colour changes to pigments and binding media (arabic gum, animal glue and egg-yolk) used in the paintings.

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