Escola Superior de Conservació i Restauració de Bés Culturals de Catalunya
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Identification of a fungal community on gilded wood carved heritage

Contributor(s): BRANDÃO, João C | JURADO, Valme | MACEDO, Maria Filomena | OLIVEIRA, Bruna P | PINHEIRO, Ana Catarina | ROSADO, Laura | VERÍSSIMO, CristinaMaterial type: ArticleArticleDescription: 6 pISBN: 1296-2074Subject(s): Biologia molecular | Cultural heritage | DHPLC | fusta | Gilded wood | madera | Moble | Molecula biology | Mueble | restauració | Restauración | Restoration | S. lacrymans In: Journal of Cultural Heritage 3 14 1, 76-81Abstract: During restoration activities, a fungal reddish area was noted on a gilded wood carved ceiling decoration from a sacristy of a church in Aveiro, Portugal. The protocols used to identify the fungal community present in this wooden material included the classical methods and molecular biology protocol based on the ability of genaturing high performance liquid chromatography (DHPLC) to separate fungal DNA from several species. This last method complemented the results obtained with the traditional culturing method approach and the overall study revealed three fungal species: Serpula lacrymans (Wulfen) P. Karsten, Paecilomyces lilacinus (Thom) Samson and Penicillum chrysogenum Thom. This is, to the autor's knowledge, the first documented case of S. lacrymans in Portuguese built cultural heritage. Despite the antifungal solution applied and the dryer environment recommended, the water activity (Aw) levels recorded on location are still dangerously high and may allow fungal regrowth. Periodic inspections were advised.
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Article de revista Article de revista Biblioteca de l' Escola Superior Conservació i Restauració de Bens Culturals de Catalunya
Journal of Cultural Heritage 3 (Browse shelf(Opens below)) Available R: 2986 Art-188

During restoration activities, a fungal reddish area was noted on a gilded wood carved ceiling decoration from a sacristy of a church in Aveiro, Portugal. The protocols used to identify the fungal community present in this wooden material included the classical methods and molecular biology protocol based on the ability of genaturing high performance liquid chromatography (DHPLC) to separate fungal DNA from several species. This last method complemented the results obtained with the traditional culturing method approach and the overall study revealed three fungal species: Serpula lacrymans (Wulfen) P. Karsten, Paecilomyces lilacinus (Thom) Samson and Penicillum chrysogenum Thom. This is, to the autor's knowledge, the first documented case of S. lacrymans in Portuguese built cultural heritage. Despite the antifungal solution applied and the dryer environment recommended, the water activity (Aw) levels recorded on location are still dangerously high and may allow fungal regrowth. Periodic inspections were advised.

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